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101.
102.
WANG Jinrong HU Yongbin HUANG Shufeng CHEN Bin YE Yuansheng LUO Xiaohua Lv Xinbiao 《《地质学报》英文版》2017,91(Z1):289-290
<正>1 Introduction Rare-metal granites are widely distributed in South China.The Daping porphyritic granitic Ta-Nb deposit,located in the Yongding area of south Fujian province,South China,is a large rare-metal deposit recently discovered.Few studies have been made of its petrology,mineralogy,geochemistry,chronology and metallogeny.In recent years,several exploratory drillings have been done in this deposit.These drilling holes,from 380 to 600 相似文献
103.
Lymphocystis disease virus (LCDV) infects target cells by attaching to a 27.8 kDa receptor (27.8R) protein in flounder Paralichthys olivaceus, and anti-27.8R monoclonal antibodies (MAbs) have been developed. However, the 27.8R existence in tissues of sea bass (Lateolabrax japonicus) and its role in LCDV infection have remained unclear. In this study, the results of western blotting demonstrated that the same 27.8R was shared by flounder and sea bass. LCDV-free sea bass individuals were intramuscularly injected with LCDV, and viral copies were detected in tissues from 3 h post infection and showed a time-dependent increase during 9 days infection. Distribution and synthesis of 27.8R in sea bass tissues were investigated by using anti-27.8R MAbs as probes. It was found that 27.8R was distributed in all the tested tissues. The levels of 27.8R protein were highest in gill and skin, then a bit lowly in stomach, head kidney and heart, followed by spleen, intestine, blood cells, gonad and liver, and least in kidney and brain in healthy sea bass. Upon LCDV infection, 27.8R synthesis was up-regulated in each tissue, and higher in the tissues with higher LCDV copies. The 27.8R and LCDV were detected in some peripheral blood leukocytes but not in red blood cells. These results suggested that 27.8R was widely distributed in sea bass tissues, and it served as a receptor and correlated with tissue tropism of LCDV infection. Furthermore, leukocytes had the potential of being a LCDV carrier and were responsible for a systemic infection of LCDV in sea bass. 相似文献
104.
We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder (Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405),
we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect
LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral
supernatant, skin nodules, gut, gill, kidney, spleen, stomach, heart, and liver of Japanese flounder. We compared the DNA
sequence with 14 MCP nucleotide sequences from GenBank, including Megalocytivirus (OFIV and RSIV), Iridovirus (CzIV and WIV), Ranavirus (TFV and FV3), and Lymphocystivirus (8 LCDV). Based on the alignment, we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH, we noted the presence of LCDV in the skin nodules, gut, gill, spleen,
stomach, and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s
black rockfish (Sebastes schlegeli Higendorf), redwing sea robin (Lepidotrigla microptera Günther) and turbot (Scophthalmus maximus) using the one-step and nested PCR, suggesting the target genes can be widely detected in fish using this method. 相似文献
105.
HCHO is ubiquitous and important chemical constitutes in the troposphere. The concentrations of the HCHO (aq) in the rainwater were measured in the Guiyang city, southeastern of China from May 2006 to April 2007 and 153 discrete samples were collected. Rainwater (N = 151) HCHO (aq) concentrations ranged from lower than method detection limit (MDL) to 40.2 µmol/L with a volume weighted mean value of 7.4 ± 8.8 µmol/L. The strong correlations between HCHO (aq) and HCOO? (r = 0.69, n = 137), HCHO (aq) and nss‐ (r = 0.74, n = 137), HCHO (aq) and (r = 0.67, n = 137), HCHO (aq) and (r = 0.74, n = 133) suggest the significant influence of the anthropogenic input for the HCHO (aq) levels. The concentration levels of rainwater HCHO (aq) was inversely proportional to the amount of rainfall, indicating the below‐cloud process is the most important mechanism for rainwater HCHO (aq) scavenging processes. More than 70% of the HCHO (aq) wet deposition took place during the early stage of the rainfall. According to the air mass back‐trajectory analysis, the rainwater with industrial back‐trajectories coming from the north had the highest levels of HCHO (aq) while the rainwater with the green‐covered or marine back‐trajectories from the southeast had the lowest concentrations, and this indicate the HCHO (aq) originated from urban or industrial regions served as an important source of the rainwater. The annual HCHO (aq) wet deposition flux was calculated as 6.96 mmol/m2 per year and the total deposition flux was estimated as 24.35 mmol/m2 per year, 71.4% of which was dominated by dry deposition. 相似文献
106.
风沙流研究越来越受到人们的重视,其中风沙流通量廓线研究起着重要作用。本文总结了3种典型地表(流沙地表、草方格地表、戈壁地表)风沙流通量廓线研究现状及存在问题。由于研究方法、研究区域及集沙效率的不同,风沙流通量廓线函数有指数函数、对数函数、幂函数、分段函数等多种,同一问题不同函数模拟结果差异可达到3倍,难以应用于实际。根据研究中存在的问题,提出风沙流通量廓线研究发展趋势。 相似文献
107.
108.
多排抗滑桩设计中的推力分担比模拟 总被引:1,自引:0,他引:1
山区铁路修建过程中,大型滑坡常采用多排抗滑桩的工程措施进行加固治理。目前常规的计算方法无法准确计算出作用在每排抗滑桩上的推力大小以及分担比。以ANSYS大型数值有限元软件为手段,以六沾线曹家山滑坡为研究对象。通过进行数值计算的方式得到作用在每排抗滑桩上的推力大小,并统计出相应每排桩所承担的滑坡下滑推力比,该方法为抗滑桩的最终结构计算提供更为科学、合理的依据,从而避免了原方法存在的种种局限性,为多排抗滑桩的设计提供了一种行之有效的解决办法。 相似文献
109.
破坏性地震应急信息处置研究现状简述 总被引:1,自引:0,他引:1
结合地震应急实例,分析了对破坏性地震应急信息处置的认识,并对国内外有关方面的研究现状进行了简要介绍. 相似文献
110.
Ying Zhang Zhenbo Lv Bo Guan Yuanjin Liu Fan Li Shaowen Li Yuanqing Ma Junbao Yu Yunzhao Li 《洁净——土壤、空气、水》2013,41(10):1027-1034
Laizhou Bay, located in the northwest of Shandong Peninsula, has complex transitional environments between terrestrial and marine ecosystems. In the present study, a total of 122, 131, and 139 species were collected in spring, summer, and autumn 2011, respectively. Species constitutions of macrobenthos were grouped into four phyla, of which annelida were the most abundant phylum, the average biomass proportion of echinodermata was the lowest, and the proportion of important species for mollusca was the highest. The structure of the macrobenthic community showed significant differences between sites, and greater divergence was observed between the third site (S03) and other stations. The ABC plots showed that the biomass curve lay below the abundance curve, and the W‐statistic value was negative. The result of the BOPA index showed that two stations had moderate ecological status in spring and that there were two heavily polluted sites and one moderately polluted site in summer. The BIO‐ENV analyses indicated that the grain‐size fractions together with trace metals (Hg, Pb, Zn, Cu, and Cr) could be considered as the major environmental variables influencing the macrobenthic patterns. The results together demonstrated that the macrobenthic communities in Laizhou Bay were negatively affected, perhaps by the tremendous impact of heavy metals in the sediments. 相似文献